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सदस्य:88gayathri.s/Swati Patankar

विकिपीडिया, मुक्‍त ज्ञानकोशातून

Dr. Swati Patankar

[संपादन]

Dr. Swati Patankar is a professor in the Department of Biosciences and Bioengineering at Indian Institute of Technology Bombay. Her main area of research is Molecular Parasitology with an emphasis on malaria.

88gayathri.s/Swati Patankar


Education

[संपादन]

Dr. Patankar received her IB diploma from Armand Hammer United World College, Montezuma, New Mexico. She went on to receive her Bachelor's degree in Life Sciences/ Biochemistry from St. Xavier’s College, Mumbai, India. She followed her academic pursuit with a Doctoral degree in Molecular Microbiology from Tufts University, Boston, USA. In her Ph.D. project, she characterized the regulation of tyrosine hydrolase gene in rats[१] [२]. She laid the foundation stone for her research career with a Postdoctoral fellowship at the Immunology & Infectious Diseases Harvard School of Public Health, Boston, USA. This was her first stint with Plasmodium[३].

Career

[संपादन]

Dr. Patankar worked as a group leader for Nicholas Piramal India Limited. Later, she joined IIT-Bombay as an Assistant Professor and rose to the rank of a Professor. She is the course instructor of Molecular Biology, Topics in Biotechnology, and Biological enquiry: History and Philosophy. She was awarded Excellence in Teaching in the years 2012 and 2016. She has been appointed as the Dean of International Relations of IIT-Bombay in 2018. She was also a visiting scientist at Harvard School of Public Health as well as Advanced Centre for Training, Research and Education in Cancer. Dr. Patankar is a lifetime member of Indian Society for Cell Biology. She has been an invited member of research and training in WHO Tropical Diseases (TDR).

Research

[संपादन]

The genome of Plasmodium falciparum genome is known to be AT-rich. This makes it difficult to identify the translation initiation sites in its genome. Dr. Patankar's team defined the optimal sequences for the same[४]. The AT-rich genome also results in high number of open reading frames (ORFs). Her research sheds light on how the expression of these regions are repressed[५]. Additionally, they found that conserved GC-rich sequences were associated with antigenic proteins of the parasite[६].


Dr. Patankar's lab has attempted to understand of RNA-mediated processes in Plasmodium falciparum. They identified putative small nuclear RNA (snRNA) and small nucleolar RNA (snoRNA) in the parasite[७]. They have worked on the trafficking of snRNAs, as well. Their work suggests that the parasite lacks the cellular machinery for trafficking of snRNAs between the nucleus and the cytoplasm. Hence, it might hypermethylated the snRNAs in the nucleus or nucleolus[८].


Dr. Patanakar worked in collaboration with Prof. Pradeepkumar P. I. on gene silencing. They found that protein phosphatase (PP2Ac) gene can be repressed by the presence of a putative G‐quadruplex (G4) ‐forming sequence. This can target fragile X mental retardation protein (FMRP)[९]. Together, they also worked on creating modified small interfering RNAs (siRNAs) that showed gene silencing in HeLa cells. They further deciphered the structural and dynamic features of this modification in siRNA duplexes[१०].

Plasmodium falciparum transports its proteins to the host cell membrane and to the apicoplast. Dr. Patankar's research elucidates the different protein trafficking pathways in the parasite. The principal pathways are: (i) the vesicular trafficking pathway that transport secretory proteins and an apicoplast membrane protein, PfTPxGl; and (ii) the pathway independent of G protein-coupled vesicles that transport apicoplast luminal proteins[११]. P. falciparum glutathione peroxidase‐like thioredoxin peroxidase (PfTPxGl) might be trafficked by the ER–Golgi pathway[१२]. They delved deeper to find that PfTPxGl possess signal anchors for this apicoplast targeting pathway[१३]. Among the P. falciparum proteins that contain a classical nuclear localization signal (NLS) are RNA hypermethylase and trimethylguanosine synthase (PfTGS1). PfTGS1 has an insertion with unknown function. Dr. Patankar's team was the first to identify the insertion as the NLS that binds to P. falciparum importin-α[१४]. Moreover, her lab investigated the unconventional mechanisms for regulation of cargo binding and release. Plasmodium falciparum was shown to have a single importin α isoform lacking auto-inhibition. This lack of auto-inhibition may aid in the faster growth the parasite[१५]. Besides this, her team studied protein trafficking in another parasite, Toxoplasma gondii. Thioredoxin peroxidase 1/2 (TgTPx1/2), in this organism, is transported to the apicoplast and the mitochondrion. How does the cell know where to transport this protein? The answer lies an ambiguous signal sequence along with a hierarchy of recognition by the protein trafficking machinery[१६].

Plasmodium falciparum lacks certain antioxidant proteins from key subcellular compartments. Dr. Patankar's research found that this absence is compensated by the cross-talk between the glutathione and thioredoxin systems[१७].

Dr. Patankar's lab also sought antimalarial compounds that targeted tubulin. They developed assays to assess the binding of lead compounds to tubulin dimer[१८]. In partnership with Prof. Brian Cooke and Prof. Ross Coppel, the team found that curcumin perturbs the microtubules P. falciparum, at least, in part[१९]. In collaboration with Prof. B. Madhusudan, they entrapped curcuminoids in lipid nanoparticles that proved to be twice as effective as free curcuminoids against malaria[२०]. Together, they also identified the antimalarial activity of antituberculosis drugs: rifampicin, isoniazide, and ethambutol[२१]. In a joint project with Rinti Banerjee and Prof. B. Madhusudan, the team formulated curcuminoids-loaded liposomes that may show antimalarial activity[२२]. Plasmepsins in Plasmodium falciparum are involved in hemoglobin (Hb) catabolism. Dr. Patankar joined hands with Dr. Prasenjit Bhaumik to make structure-guided drug discovery of inhibitors that block plasmepsins. They resolved the crystal structures of plasmepsin-inhibitor complexes revealing features that modulate their differential potency[२३].


Dr. Patankar worked with Prof. Sanjeeva Srivastava on various proteomics studies of malaria. The group tried to understand the antibody responses to P. falciparum and P. vivax in Southwest India[२४]. Additionally, they conducted a multi-disciplinary study to find potential predictive markers for malaria severity[२५].

References

[संपादन]
  1. ^ Lazaroff, Meredith; Patankar, Swati; Yoon, Sung Ok; Chikaraishi, Dona M. (1995-09-15). "The Cyclic AMP Response Element Directs Tyrosine Hydroxylase Expression in Catecholaminergic Central and Peripheral Nervous System Cell Lines from Transgenic Mice". Journal of Biological Chemistry (इंग्रजी भाषेत). 270 (37): 21579–21589. doi:10.1074/jbc.270.37.21579. ISSN 0021-9258.
  2. ^ Patankar, Swati; Lazaroff, Meredith; Yoon, Sung Ok; Chikaraishi, Dona M. (1997-06-01). "A Novel Basal Promoter Element Is Required for Expression of the Rat Tyrosine Hydroxylase Gene". Journal of Neuroscience (इंग्रजी भाषेत). 17 (11): 4076–4086. doi:10.1523/JNEUROSCI.17-11-04076.1997. ISSN 0270-6474. PMC 6573541. PMID 9151724.CS1 maint: PMC format (link)
  3. ^ Patankar, Swati; Fujioka, Hisashi; Wirth, Dyann F (2000-12-01). "The signal sequence and C-terminal hydrophobic domain are required for localization of the sexual stage antigen Pgs28 to the surface of P. gallinaceum ookinetes". Molecular and Biochemical Parasitology (इंग्रजी भाषेत). 111 (2): 425–435. doi:10.1016/S0166-6851(00)00340-6. ISSN 0166-6851.
  4. ^ Patakottu, Balakota Reddy; Singh, Prashant Kumar; Malhotra, Pawan; Chauhan, V. S.; Patankar, Swati (2012-03). "In vivo analysis of translation initiation sites in Plasmodium falciparum". Molecular Biology Reports (इंग्रजी भाषेत). 39 (3): 2225–2232. doi:10.1007/s11033-011-0971-3. ISSN 0301-4851. |date= मधील दिनांक मूल्ये तपासा (सहाय्य)
  5. ^ Kumar, Mayank; Srinivas, Vivek; Patankar, Swati (2015-12). "Upstream AUGs and upstream ORFs can regulate the downstream ORF in Plasmodium falciparum". Malaria Journal (इंग्रजी भाषेत). 14 (1): 512. doi:10.1186/s12936-015-1040-5. ISSN 1475-2875. PMC 4687322. PMID 26692187. |date= मधील दिनांक मूल्ये तपासा (सहाय्य)CS1 maint: PMC format (link)
  6. ^ Panneerselvam, Porkodi; Bawankar, Praveen; Kulkarni, Surashree; Patankar, Swati (2011-01). "In Silico Prediction of Evolutionarily Conserved GC-Rich Elements Associated with Antigenic Proteins of Plasmodium falciparum". Evolutionary Bioinformatics (इंग्रजी भाषेत). 7: EBO.S8162. doi:10.4137/EBO.S8162. ISSN 1176-9343. PMC 3283219. PMID 22375094. |date= मधील दिनांक मूल्ये तपासा (सहाय्य)CS1 maint: PMC format (link)
  7. ^ Upadhyay, Roopam; Bawankar, Praveen; Malhotra, Deepti; Patankar, Swati (2005-12). "A screen for conserved sequences with biased base composition identifies noncoding RNAs in the A–T rich genome of Plasmodium falciparum". Molecular and Biochemical Parasitology (इंग्रजी भाषेत). 144 (2): 149–158. doi:10.1016/j.molbiopara.2005.08.012. |date= मधील दिनांक मूल्ये तपासा (सहाय्य)
  8. ^ Bawankar, Praveen; Shaw, Philip J.; Sardana, Richa; Babar, Prasad H.; Patankar, Swati (2010-04). "5′ and 3′ end modifications of spliceosomal RNAs in Plasmodium falciparum". Molecular Biology Reports (इंग्रजी भाषेत). 37 (4): 2125–2133. doi:10.1007/s11033-009-9682-4. ISSN 0301-4851. |date= मधील दिनांक मूल्ये तपासा (सहाय्य)
  9. ^ Pany, Sushree Prangya P.; Sapra, Mahak; Sharma, Jitendar; Dhamodharan, V.; Patankar, Swati; Pradeepkumar, P. I. (2019-12-02). "Presence of Potential G‐Quadruplex RNA‐Forming Motifs at the 5′‐UTR of PP2Acα mRNA Repress Translation". ChemBioChem (इंग्रजी भाषेत). 20 (23): 2955–2960. doi:10.1002/cbic.201900336. ISSN 1439-4227.
  10. ^ Gore, Kiran R.; Nawale, Ganesh N.; Harikrishna, S.; Chittoor, Vinita G.; Pandey, Sushil Kumar; Höbartner, Claudia; Patankar, Swati; Pradeepkumar, P. I. (2012-04-06). "Synthesis, Gene Silencing, and Molecular Modeling Studies of 4′- C -Aminomethyl-2′- O -methyl Modified Small Interfering RNAs". The Journal of Organic Chemistry (इंग्रजी भाषेत). 77 (7): 3233–3245. doi:10.1021/jo202666m. ISSN 0022-3263.
  11. ^ Chaudhari, Rahul; Dey, Vishakha; Narayan, Aishwarya; Sharma, Shobhona; Patankar, Swati (2017-04-27). "Membrane and luminal proteins reach the apicoplast by different trafficking pathways in the malaria parasite Plasmodium falciparum". PeerJ (इंग्रजी भाषेत). 5: e3128. doi:10.7717/peerj.3128. ISSN 2167-8359. PMC 5410153. PMID 28462015.CS1 maint: PMC format (link)
  12. ^ Chaudhari, Rahul; Narayan, Aishwarya; Patankar, Swati (2012-10). "A novel trafficking pathway in Plasmodium falciparum for the organellar localization of glutathione peroxidase-like thioredoxin peroxidase". FEBS Journal (इंग्रजी भाषेत). 279 (20): 3872–3888. doi:10.1111/j.1742-4658.2012.08746.x. |date= मधील दिनांक मूल्ये तपासा (सहाय्य)
  13. ^ Narayan, Aishwarya; Mastud, Pragati; Thakur, Vandana; Rathod, Pradipsinh K.; Mohmmed, Asif; Patankar, Swati (2018-11). "Heterologous expression in Toxoplasma gondii reveals a topogenic signal anchor in a Plasmodium apicoplast protein". FEBS Open Bio (इंग्रजी भाषेत). 8 (11): 1746–1762. doi:10.1002/2211-5463.12527. PMC 6212639. PMID 30410855. |date= मधील दिनांक मूल्ये तपासा (सहाय्य)CS1 maint: PMC format (link)
  14. ^ Babar, Prasad H.; Dey, Vishakha; Jaiswar, Praveen; Patankar, Swati (2016-11-01). "An insertion in the methyltransferase domain of P. falciparum trimethylguanosine synthase harbors a classical nuclear localization signal". Molecular and Biochemical Parasitology (इंग्रजी भाषेत). 210 (1): 58–70. doi:10.1016/j.molbiopara.2016.08.007. ISSN 0166-6851.
  15. ^ Dey, Vishakha; Patankar, Swati (2018-09). "Molecular basis for the lack of auto-inhibition of Plasmodium falciparum importin α". Biochemical and Biophysical Research Communications (इंग्रजी भाषेत). 503 (3): 1792–1797. doi:10.1016/j.bbrc.2018.07.115. |date= मधील दिनांक मूल्ये तपासा (सहाय्य)
  16. ^ Mastud, Pragati; Patankar, Swati (2019-07-18). "An ambiguous N-terminus drives the dual targeting of an antioxidant protein Thioredoxin peroxidase (TgTPx1/2) to endosymbiotic organelles in Toxoplasma gondii". PeerJ (इंग्रजी भाषेत). 7: e7215. doi:10.7717/peerj.7215. ISSN 2167-8359. PMC 6642795. PMID 31346496.CS1 maint: PMC format (link)
  17. ^ Chaudhari, Rahul; Sharma, Shobhona; Patankar, Swati (2017-06). "Glutathione and thioredoxin systems of the malaria parasite Plasmodium falciparum : Partners in crime?". Biochemical and Biophysical Research Communications (इंग्रजी भाषेत). 488 (1): 95–100. doi:10.1016/j.bbrc.2017.05.015. |date= मधील दिनांक मूल्ये तपासा (सहाय्य)
  18. ^ Chakrabarti, Rimi; Patankar, Swati (2016-10-14). "Combination Assays and Molecular Docking can Identify Binding sites of Anti-Microtubule Drugs on Plasmodium falciparum Tubulin". Infectious Disorders - Drug Targets (इंग्रजी भाषेत). 16 (3): 204–216. doi:10.2174/1871526516666160711164905.
  19. ^ Chakrabarti, Rimi; Rawat, Parkash S.; Cooke, Brian M.; Coppel, Ross L.; Patankar, Swati (2013-03-07). Bejon, Philip (ed.). "Cellular Effects of Curcumin on Plasmodium falciparum Include Disruption of Microtubules". PLoS ONE (इंग्रजी भाषेत). 8 (3): e57302. doi:10.1371/journal.pone.0057302. ISSN 1932-6203. PMC 3591428. PMID 23505424.CS1 maint: PMC format (link)
  20. ^ Nayak, Aditya P.; Tiyaboonchai, Waree; Patankar, Swati; Madhusudhan, Basavaraj; Souto, Eliana B. (2010-11). "Curcuminoids-loaded lipid nanoparticles: Novel approach towards malaria treatment". Colloids and Surfaces B: Biointerfaces (इंग्रजी भाषेत). 81 (1): 263–273. doi:10.1016/j.colsurfb.2010.07.020. |date= मधील दिनांक मूल्ये तपासा (सहाय्य)
  21. ^ Aditya, Nayak P.; Patankar, Swati; Madhusudhan, Basavaraj (2010-05). "Assessment of in vivo antimalarial activity of rifampicin, isoniazide, and ethambutol combination therapy". Parasitology Research (इंग्रजी भाषेत). 106 (6): 1481–1484. doi:10.1007/s00436-010-1789-y. ISSN 0932-0113. |date= मधील दिनांक मूल्ये तपासा (सहाय्य)
  22. ^ Aditya, N.P.; Chimote, Geetanjali; Gunalan, Karthigayan; Banerjee, Rinti; Patankar, Swati; Madhusudhan, Basavaraj (2012-07). "Curcuminoids-loaded liposomes in combination with arteether protects against Plasmodium berghei infection in mice". Experimental Parasitology (इंग्रजी भाषेत). 131 (3): 292–299. doi:10.1016/j.exppara.2012.04.010. |date= मधील दिनांक मूल्ये तपासा (सहाय्य)
  23. ^ Mishra, Vandana; Rathore, Ishan; Arekar, Anagha; Sthanam, Lakshmi Kavitha; Xiao, Huogen; Kiso, Yoshiaki; Sen, Shamik; Patankar, Swati; Gustchina, Alla; Hidaka, Koushi; Wlodawer, Alexander (2018-08). "Deciphering the mechanism of potent peptidomimetic inhibitors targeting plasmepsins – biochemical and structural insights". The FEBS Journal (इंग्रजी भाषेत). 285 (16): 3077–3096. doi:10.1111/febs.14598. ISSN 1742-464X. PMC 6309945. PMID 29943906. |date= मधील दिनांक मूल्ये तपासा (सहाय्य)CS1 maint: PMC format (link)
  24. ^ Venkatesh, Apoorva; Jain, Aarti; Davies, Huw; Periera, Ligia; Maki, Jennifer N.; Gomes, Edwin; Felgner, Philip L.; Srivastava, Sanjeeva; Patankar, Swati; Rathod, Pradipsinh K. (2019-12). "Hospital-derived antibody profiles of malaria patients in Southwest India". Malaria Journal (इंग्रजी भाषेत). 18 (1): 138. doi:10.1186/s12936-019-2771-5. ISSN 1475-2875. PMC 6472095. PMID 30995911. |date= मधील दिनांक मूल्ये तपासा (सहाय्य)CS1 maint: PMC format (link)
  25. ^ Ris, M. M.; Deitrich, R. A.; Von Wartburg, J. P. (1975-10-15). "Inhibition of aldehyde reductase isoenzymes in human and rat brain". Biochemical Pharmacology. 24 (20): 1865–1869. doi:10.1016/0006-2952(75)90405-0. ISSN 0006-2952. PMID 18.
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